高效液相色谱法测定氯霉素滴眼液中硫柳汞和苯基汞的含量

易大为, 马琳, 张亚杰

中国药学杂志 ›› 2014, Vol. 49 ›› Issue (11) : 1004-1008.

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中国药学杂志 ›› 2014, Vol. 49 ›› Issue (11) : 1004-1008. DOI: 10.11669/cpj.2014.11.023
论 著

高效液相色谱法测定氯霉素滴眼液中硫柳汞和苯基汞的含量

  • 易大为, 马琳, 张亚杰
作者信息 +

Determination of Thimerosal and Phenylmercuric Compounds in Chloramphenicol Eye Drops by HPLC

  • YI Da-wei, MA Lin, ZHANG Ya-jie
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文章历史 +

摘要

目的 建立氯霉素滴眼液中硫柳汞和苯基汞防腐剂的含量测定方法。方法 采用高效液相色谱法, 色谱柱为Agilent 苯基柱, 0.05 mol·L-1磷酸二氢钾溶液(用三乙胺调节pH值至5.5)-乙腈(82∶18)为流动相, 流速为1.2 mL·min-1, 检测波长为220 nm。结果 硫柳汞和醋酸苯汞在2~100 μg·mL-1之间呈良好的线性关系(r>0.999 0), 硫柳汞的平均回收率≥96.9%, 醋酸苯汞的平均回收率≥99.1%。结论 本法简便、准确、专属性强, 可用于药品中有机汞防腐剂的控制。

Abstract

OBJECTIVE To develop an HPLC method for simultaneous determination of thimerosal and phenylmercuric compounds in chloramphenicol eye drops. METHODS The analysis was performed on a phenyl-column with an ion-suppress mobile phase system consisting of 0.05 mol·L-1 potassium dihydrogen phosphate solution(pH adjusted to 5.5 with triethylamine)/acetonitrile(82∶18, V/V)at a flow rate of 1.2 mL·min-1 at 220 nm. RESULTS The established method had good linearity within the concentration range of 2.0-100 μg·mL-1(r>0.999 0)for thimerosal and phenylmercuric acetate, with average recoveries of ≥96.9% for thimerosal and ≥99.1% for phenylmercuric acetate. CONCLUSION The method is simple, accurate and specific for determination of thimerosal and phenylmercuric compounds in chloramphenicol eye drops.

关键词

高效液相色谱法 / 苯基柱 / 硫柳汞 / 苯基汞化合物 / 氯霉素滴眼液

Key words

HPLC / phenyl-column / thimerosal / phenylmercuric compound / chloramphenicol eye drop

引用本文

导出引用
易大为, 马琳, 张亚杰. 高效液相色谱法测定氯霉素滴眼液中硫柳汞和苯基汞的含量[J]. 中国药学杂志, 2014, 49(11): 1004-1008 https://doi.org/10.11669/cpj.2014.11.023
YI Da-wei, MA Lin, ZHANG Ya-jie. Determination of Thimerosal and Phenylmercuric Compounds in Chloramphenicol Eye Drops by HPLC[J]. Chinese Pharmaceutical Journal, 2014, 49(11): 1004-1008 https://doi.org/10.11669/cpj.2014.11.023
中图分类号: R917   

参考文献

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